Prep HPLC Column, RP C4, Wide Pore, 10um, 300A. 30mm ID x 50mm Length. Cogent Brand. 1 EA.

Additional Information:

Cogent RP C4 Butyl Wide Pore is a reversed-phase HPLC stationary phase manufactured using high-purity silica with traditional butyl (C4) ligand bonding and complete double endcapping. The 300 Å pore structure is specifically engineered to improve accessibility for proteins, peptides, enzymes, and other large biomolecules, making it well suited for preparative purification applications where high recovery and efficient mass transfer are essential.

68004-P30-50-3M is a preparative HPLC column with dimensions of 30 mm ID x 50 mm length, packed with 10 µm RP C4 Butyl Wide Pore particles featuring a 300 Å pore size. The large internal diameter provides substantial sample loading capacity and efficient fraction collection, while the short 50 mm length is optimized for rapid purification cycles, method scouting, and process development. Typical flow rates are approximately 30–80 mL/min depending on system capabilities, mobile phase conditions, and purification requirements.

This column is specifically intended for preparative purification of proteins, peptides, enzymes, and other large hydrophobic or neutral biomolecules. The combination of wide pore architecture, fully endcapped C4 chemistry, and preparative dimensions provides excellent analyte recovery and reproducible purification performance. The shorter column format is particularly advantageous when throughput and purification speed are prioritized over maximum chromatographic resolution. A specialized preparative HPLC system capable of high flow rates, large injection volumes, and fraction collection is required. This product is supplied as a Special Order Item manufactured specifically upon receipt of order.

Key Benefits

  • RP C4 Butyl Wide Pore stationary phase

  • 300 Å pore size optimized for biomolecules

  • Fully double endcapped chemistry

  • Traditional butyl (C4) bonded phase

  • Designed for preparative HPLC purification

  • Excellent recovery of proteins and peptides

  • High sample loading capacity

  • 10 µm particles for lower operating backpressure

  • Suitable for high flow rates

  • Fast purification cycles with 50 mm column length

Typical Applications

  • Protein purification

  • Peptide purification

  • Enzyme purification

  • Biomolecule isolation

  • Biopharmaceutical research

  • Biotechnology research

  • Preparative HPLC purification

  • Process development

  • Method scouting

  • Fraction collection

Specifications

  • Product Type: Preparative HPLC Column

  • Stationary Phase: RP C4 Butyl Wide Pore

  • Chromatography Mode: Reversed Phase

  • Bonded Phase: Butyl (C4)

  • Endcapping: Fully Double Endcapped

  • Particle Size: 10 µm

  • Pore Size: 300 Å

  • Column Dimensions: 30 mm ID x 50 mm

  • Length: 50 mm

  • Typical Flow Rate: 30–80 mL/min

  • Brand: Cogent

Special Order Item Information

Important Notes

  • Designed for purification rather than routine analytical HPLC

  • Intended for collection and isolation of purified biomolecules

  • Optimized for proteins, peptides, enzymes, and other large molecules

  • Requires a preparative HPLC system capable of high flow rates and fraction collection

  • Wide pore structure improves accessibility and recovery of biomolecules

  • Short column length supports rapid purification and method development workflows

  • Special Order Item


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